gallic acid Search Results


93
MedChemExpress gallic acid
Gallic Acid, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Chem Impex International gallic acid
Gallic Acid, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Valiant Co Ltd n propyl gallate
N Propyl Gallate, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
TargetMol ml385 t4360
Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor <t>ML385</t> (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.
Ml385 T4360, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
ChromaDex theaflavin 3 gallate
Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor <t>ML385</t> (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.
Theaflavin 3 Gallate, supplied by ChromaDex, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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91
ChromaDex gaa
Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor <t>ML385</t> (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.
Gaa, supplied by ChromaDex, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
ChromaDex gallocatechin 3 gallate
Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor <t>ML385</t> (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.
Gallocatechin 3 Gallate, supplied by ChromaDex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
ChromaDex syringic acid
Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor <t>ML385</t> (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.
Syringic Acid, supplied by ChromaDex, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Valiant Co Ltd gallic acid
Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor <t>ML385</t> (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.
Gallic Acid, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gallic acid/product/Valiant Co Ltd
Average 93 stars, based on 1 article reviews
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93
Selleck Chemicals ga s4603 selleck
Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor <t>ML385</t> (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.
Ga S4603 Selleck, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
ChromaDex gallocatechin gallate
Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor <t>ML385</t> (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.
Gallocatechin Gallate, supplied by ChromaDex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Santa Cruz Biotechnology gallic acid
Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor <t>ML385</t> (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.
Gallic Acid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gallic acid/product/Santa Cruz Biotechnology
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Image Search Results


Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor ML385 (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.

Journal: Frontiers in Immunology

Article Title: Gallic Acid Alleviates Gouty Arthritis by Inhibiting NLRP3 Inflammasome Activation and Pyroptosis Through Enhancing Nrf2 Signaling

doi: 10.3389/fimmu.2020.580593

Figure Lengend Snippet: Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor ML385 (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.

Article Snippet: Gallic acid (T0877), colchicine ((T0320), and ML385 (T4360) were acquired from TargetMol (Boston, MA, USA).

Techniques: Activation Assay, Derivative Assay, Incubation, Immunofluorescence, Staining, Fluorescence, Western Blot, Enzyme-linked Immunosorbent Assay, Immunoprecipitation

Gallic acid decreases monosodium urate (MSU)-induced NLRP3 inflammasome activation partly through Nrf2. (A – E) LPS-primed BMDMs were treated with ML385 for 30 min, incubated with gallic acid for 30 min, and then stimulated with MSU for 6 h. (A) Representative IF images of cell death indicated by PI and Hoechst 33342 staining. The percentage of PI-positive cells relative to all cells was calculated; 10 randomly chosen fields were quantified. (B) Representative IF images of MitoTracker-stained BMDMs. Scale bars, 10 μm. The relative fluorescence intensity of MitoTracker was compared with that of control BMDMs. (C) The culture supernatant was obtained to analyze LDH release. (D) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by immunoblotting. (E, F) ELISA kit was used to detect IL-1β levels in culture supernatants. GA, gallic acid. ** P < 0.01, *** P < 0.001.

Journal: Frontiers in Immunology

Article Title: Gallic Acid Alleviates Gouty Arthritis by Inhibiting NLRP3 Inflammasome Activation and Pyroptosis Through Enhancing Nrf2 Signaling

doi: 10.3389/fimmu.2020.580593

Figure Lengend Snippet: Gallic acid decreases monosodium urate (MSU)-induced NLRP3 inflammasome activation partly through Nrf2. (A – E) LPS-primed BMDMs were treated with ML385 for 30 min, incubated with gallic acid for 30 min, and then stimulated with MSU for 6 h. (A) Representative IF images of cell death indicated by PI and Hoechst 33342 staining. The percentage of PI-positive cells relative to all cells was calculated; 10 randomly chosen fields were quantified. (B) Representative IF images of MitoTracker-stained BMDMs. Scale bars, 10 μm. The relative fluorescence intensity of MitoTracker was compared with that of control BMDMs. (C) The culture supernatant was obtained to analyze LDH release. (D) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by immunoblotting. (E, F) ELISA kit was used to detect IL-1β levels in culture supernatants. GA, gallic acid. ** P < 0.01, *** P < 0.001.

Article Snippet: Gallic acid (T0877), colchicine ((T0320), and ML385 (T4360) were acquired from TargetMol (Boston, MA, USA).

Techniques: Activation Assay, Incubation, Staining, Fluorescence, Western Blot, Enzyme-linked Immunosorbent Assay