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Image Search Results
Journal: Frontiers in Immunology
Article Title: Gallic Acid Alleviates Gouty Arthritis by Inhibiting NLRP3 Inflammasome Activation and Pyroptosis Through Enhancing Nrf2 Signaling
doi: 10.3389/fimmu.2020.580593
Figure Lengend Snippet: Gallic acid suppresses nigericin-induced NLRP3 inflammasome activation and pyroptosis dependent on Nrf2. (A – F) Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages (BMDMs) were incubated with Nrf2 inhibitor ML385 (2 μM) for 30 min, treated with gallic acid (80 μM) for 30 min, and then stimulated with nigericin (10 μM). (A) Representative immunofluorescence images of MitoSOX and MitoTracker-stained BMDMs. Scale bars, 10 μm. (B) The relative fluorescence intensity of MitoSOX and MitoTracker was compared with that of control BMDMs. (E) The percentage of PI-positive cells relative to total cells was calculated; 10 randomly chosen fields were quantified. Data are shown as means ± sem ( n = 10). (C) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by western blotting. (D) ELISA of IL-1β levels in supernatants. (F) Co-Immunoprecipitation was applied to analyze the interaction between NEK7 and NLRP3. GA, gallic acid. ** P < 0.01, *** P < 0.001.
Article Snippet: Gallic acid (T0877), colchicine ((T0320), and
Techniques: Activation Assay, Derivative Assay, Incubation, Immunofluorescence, Staining, Fluorescence, Western Blot, Enzyme-linked Immunosorbent Assay, Immunoprecipitation
Journal: Frontiers in Immunology
Article Title: Gallic Acid Alleviates Gouty Arthritis by Inhibiting NLRP3 Inflammasome Activation and Pyroptosis Through Enhancing Nrf2 Signaling
doi: 10.3389/fimmu.2020.580593
Figure Lengend Snippet: Gallic acid decreases monosodium urate (MSU)-induced NLRP3 inflammasome activation partly through Nrf2. (A – E) LPS-primed BMDMs were treated with ML385 for 30 min, incubated with gallic acid for 30 min, and then stimulated with MSU for 6 h. (A) Representative IF images of cell death indicated by PI and Hoechst 33342 staining. The percentage of PI-positive cells relative to all cells was calculated; 10 randomly chosen fields were quantified. (B) Representative IF images of MitoTracker-stained BMDMs. Scale bars, 10 μm. The relative fluorescence intensity of MitoTracker was compared with that of control BMDMs. (C) The culture supernatant was obtained to analyze LDH release. (D) Supernatants (Sup.) and cell lysates (Lys.) were analyzed by immunoblotting. (E, F) ELISA kit was used to detect IL-1β levels in culture supernatants. GA, gallic acid. ** P < 0.01, *** P < 0.001.
Article Snippet: Gallic acid (T0877), colchicine ((T0320), and
Techniques: Activation Assay, Incubation, Staining, Fluorescence, Western Blot, Enzyme-linked Immunosorbent Assay